检出限
化学
分析物
纳米团簇
荧光
猝灭(荧光)
分析化学(期刊)
微流控
光电子学
纳米技术
光学
色谱法
材料科学
物理
有机化学
作者
Lipeng Pan,Mingye Zou,Fangxing Ma,Lingqing Kong,Changnan Zhang,Likun Yang,Anna Zhu,Feng Long,Xiangyang Li,Naibo Lin
标识
DOI:10.1016/j.aca.2021.339312
摘要
A compact evanescent wave detection platform (EWDP) is developed for the detection of fluorescence gold nanoclusters. The EWDP employs a simple optical system and a Si-based photodetector SOP-1000 assembly to improve the optical efficiency and detection sensitivity. A microfluidic sample cell is also used to decrease the amount of analyte to 200 μL (The volume of sample cell is really about 30 μL). On this basis, we design a strategy for detecting dopamine (DA) based on the photoinduced electron transfer (PET) quenching mechanism. By introduction of tyrosinase (TYR) during the detection, the testing time is shortened to 1 min. The fluorescence emission signal decreased dramatically and the quenching ratio (F0-F)/F0 is linearly related to the concentration of DA in the range of 0.03-60 μM with a detection limit of 0.03 μM. Additionally, this detection platform has potential applications for DA fast detection in the microsamples.
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