乳酸菌
免疫系统
拟杆菌
肠道菌群
微生物学
化学
毛螺菌科
生物
免疫学
细菌
生物化学
16S核糖体RNA
遗传学
发酵
基因
厚壁菌
作者
Haiyang Xie,Jingyu Fang,Mohamed A. Farag,Zhenhao Li,Peilong Sun,Penghui Shao
标识
DOI:10.1016/j.fochx.2022.100235
摘要
In this study, the polysaccharides extracted from Dendrobium officinale leaf (DOLP) was used in immune deficiency mice to evaluate the bioactivity. Thymus and spleen indices were calculated while the alleviation of the colon and liver histopathological progression was evaluated by H&E staining. The data indicated that DOLP improved immunity status by restoring the gut barrier and atrophy of immune organs. Cytokines levels as marker of inflammation were determined using ELISA in serum and colon. Which proved that DOLP inhibited the expression of pro-inflammatory cytokines (TNF-α, TGF- β1, IL-6, IL-1β) and promoted the expression of anti-inflammatory cytokines (IL-10). Short chain fatty acids (SCFAs) levels and microbial composition in feces were determined using GS and high-throughput sequencing. DOLP improved gut microbiota by increasing the relative abundance of total bacteria and probiotics such as Bacteroides, Lactobacillus and Lachnospiraceae. Therefore, DOLP has potential effect for the treatment of chronic immune diseases.
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