Long Noncoding RNA lnc-TCEA1-3 Affects Osteoclastic Function by Regulating ATP6V1H

H subunit of V-ATPase (ATP6V1H) is specifically expressed in osteoclasts and its deficiency lead to osteoporosis. Our group previously found four intronic SNPs of <i>ATP6V1H</i> related to reduced bone mineral density, but the mechanisms was not clear. In this study, we found that the above four SNPs were located at lncRNA<i> lnc-TCEA1-3</i> by using bioinformatics analysis. We further detected the function of<i> lnc-TCEA1-3</i> on regulating<i> ATP6V1H </i>and osteoclast function using<i> Atp6v1h</i> knockout mice, lentivirus transfection and qPCR analysis. Over expression of<i> lnc-TCEA1-3</i> up regulated the expression of <i>ATP6V1H</i> in HEK293 cells, HOS cells and primarily cultured osteoclasts, and increased the number of primarily cultured osteoclasts. In addition, over expression of<i> lnc-TCEA1-3</i> exerted distinct effect on two transcripts of <i>ATP6V1H</i> in HEK293, HOS and osteoclasts. This study will facilitate the in-depth analysis of the effects of<i> ATP6V1H</i> on bone diseases, and discover new therapeutic strategies.