生物
枯萎病
西番莲
西番莲
镰刀菌
尖孢镰刀菌
枯萎病
系统发育树
真菌学
病菌
植物
真菌
园艺
微生物学
基因
遗传学
作者
Claudia Salazar,Omar Yela-Caicedo,Bladimir Gomez-Espinoza
出处
期刊:Revista de Ciencias Agrícolas
日期:2022-06-01
卷期号:39 (2): 33-46
标识
DOI:10.22267/rcia.223902.180
摘要
In Colombia, vascular rotting of passion fruit (P. ligularis) caused by the fungus Fusarium is considered the most important pathological problem in the cultivation of this fruit. Plants affected by the pathogen wilt and die, causing considerable yield losses and increased production costs. The objective of this work was to determine the genetic variability of the Fusarium species associated with this disease. For this, 50 samples were collected from producing farms in three municipalities of the department of Nariño. A total of 35 isolates were characterized morphologically and molecularly. The morphological identification was carried out using taxonomic keys. Molecular characterization was performed by PCR with primers ITS1 – ITS4 and TEF1α. The PCR products were sent to Macrogen (Korea) for sequencing. Sequencing results indicated that Fusarium oxysporum f.sp. passiflorae is associated as a causal agent of the disease. In addition, the presence of Ilyonectria robusta was determined, which, has not been reported before in passion fruit plants. The phylogenetic analysis allowed determine that there was little variability among the isolates evaluated; no grouping was observed associated to the geographical origin of the samples. The morphological characters corroborated the species information obtained from the molecular analysis.
科研通智能强力驱动
Strongly Powered by AbleSci AI