Lotus resistance against Ralstonia is enhanced by Mesorhizobium and does not impair mutualism

Legumes establish nitrogen-fixing symbioses with rhizobia. On the contrary, they can be attacked concomitantly by pathogens, raising the question of potential trade-offs between mutualism and immunity. In order to study such trade-offs, we used a tripartite system involving the model legume Lotus japonicus, its rhizobial symbiont Mesorhizobium loti and the soilborne pathogen Ralstonia solanacearum. We investigated the impact of mutualism on plant defense and the reciprocal influence of plant defense on mutualism. We found that Lotus age-related resistance against Ralstonia was improved by the interaction with rhizobia especially when nodulation is triggered. Conversely, age-related resistance did not compromise nodule organogenesis or functioning under pathogen attack. Proteomic characterization indicates that this resistance is associated with distinct proteome modifications in roots and nodules. This resistance questions the concept of interference between efficient defense reactions and mutualistic interactions and is of great interest for agricultural purposes as it not only restricts pathogen colonization, but would also preserve nitrogen fixation and yield.