生物膜
光动力疗法
核梭杆菌
聚集放线菌
氯
牙龈卟啉单胞菌
抗菌剂
光敏剂
化学
微生物学
辐照
牙周炎
变形链球菌
慢性牙周炎
细菌
核化学
医学
生物
光化学
牙科
物理
有机化学
核物理学
遗传学
作者
Gabriel Garcia de Carvalho,Rafaela Pacheco Mateo,Rodrigo Costa e Silva,Patricia Milagros Maquera-Huacho,Alessandra Nara de Souza Rastelli,Kleber T. de Oliveira,Rosemary Adriana Chierici Marcantonio,Daniela Leal Zandim-Barcelos,Denise Madalena Palomari Spolidorio
标识
DOI:10.1016/j.pdpdt.2022.103219
摘要
In our previous study, Chlorin-e6 (Ce6) demonstrated a significant reduction of microorganisms' viability against single-species biofilm related to periodontitis once irradiated by red light (660 nm). Also, higher bacteria elimination was observed under blue light (450 nm) irradiation. However, the use of blue light irradiation of Ce6 for antimicrobial administration is poorly explored. This study evaluated the effect of chlorin-e6-mediated antimicrobial photodynamic therapy (aPDT) using different wavelengths (450 or 660 nm) against multi-species biofilms related to periodontitis. Streptococcus oralis, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans composed the mature biofilm developed under proper conditions for five days. aPDT was performed using different concentrations of Ce6 (100 and 200 μM), wavelengths (450 or 660 nm), and comparisons were made after qPCR assay and confocal laser scanning microscopy (CLSM) analysis. The greatest bacterial elimination was observed in the groups where Ce6 was used with blue light, for S. orallis (2.05 Log10 GeQ mL-1, p < 0.0001) and P. gingivalis (1.4 Log10 GeQ mL-1, p < 0.0001), aPDT with red light showed significant bacteria reduction only for S. orallis. aPDT with blue light demonstrated statistically higher elimination in comparison with aPDT with red light. The aPDT did not show a statistically significant effect when tested against A. actinomycetemcomitans and F. nucleatum (p=0.776 and 0.988, respectively). The aPDT using blue light showed a promising higher photobiological effect, encouraging researchers to consider it in the irradiation of Ce6 for further investigations.
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