Overview of online two‐dimensional liquid chromatography based on cell membrane chromatography for screening target components from traditional Chinese medicines

Cell membrane chromatography is a simple, specific, and time‐saving technique for studying drug–receptor interactions, screening of active components from complex mixtures, and quality control of traditional Chinese medicines. However, the short column life, low sensitivity, low column efficiency (so cannot resolve satisfactorily mixture of compounds), low peak capacity, and inefficient in structure identification were bottleneck in its application. Combinations of cell membrane chromatography with multidimensional chromatography such as two‐dimensional liquid chromatography and high sensitivity detectors like mass have significantly reduced many of the above‐mentioned shortcomings. This paper provides an overview of the current advances in online two‐dimensional‐based cell membrane chromatography for screening target components from traditional Chinese medicines with particular emphasis on the instrumentation, preparation of cell membrane stationary phase, advantages, and disadvantages compared to alternative approaches. The last section of the review summarizes the applications of the online two‐dimensional high‐performance liquid chromatography based cell membrane chromatography reported since its emergence to date (2010–June 2016).