化学
生物催化
酶
生物化学
药品
基质(水族馆)
生物合成
天然产物
链霉菌
细菌
组合化学
催化作用
反应机理
药理学
生物
遗传学
生态学
作者
Jiawei Guo,Fengwei Li,Fangyuan Cheng,Li Ma,Xiaohui Liu,Pradeepraj Durairaj,Gang Zhang,Dandan Tang,Xiangtian Long,Wei Zhang,Lei Du,Qizhou Zhang,Shengying Li
标识
DOI:10.1021/acs.joc.1c01407
摘要
Human drug metabolites (HDMs) are important chemicals widely used in drug-related studies. However, acquiring these enzyme-derived and regio-/stereo-selectively modified compounds through chemical approaches is complicated. PikC is a biosynthetic P450 enzyme involved in pikromycin biosynthesis from the bacterium Streptomyces venezuelae. Here, we identify the mutant PikCD50N as a potential biocatalyst, with a broad substrate scope, diversified product profile, and high catalytic efficiency, for preparation of HDMs. Remarkably, PikCD50N can mediate the drug-metabolizing reactions using the low-cost H2O2 as a direct electron and oxygen donor.
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