Recording L-type calcium channel current by perforated patch clamp and effect of dehydrocorydaline on L-type calcium channel

Aim To observe the difference of recording L-type calcium channel currents over time through by whole cell patch clamp and perforated patch clamp method and the effect of dehydrocorydaline on L-type calcium channel by perforated patch clamp method.Methods Whole cell patch clamp and perforated patch clamp were used to record L-type calcium channel current in acutely isolated rat ventricular myocytes.Results The L-type calcium channel current peak recorded by whole cell patch clamp decayed of the(34±23)%(n=10) within 15 minutes,while using perforated patch clamp,L-type calcium channel current peak attenuated(2.7±3.4)%(n=9) within 15 minutes;The inhibitory effect of ginsenoside Re(100 μmol·L-1) could be clearly recorded by perforated patch clamp method,while the current decaying produced by whole cell patch clamp almost completely overshadowed the effects of ginsenoside Re.Dehydrocorydaline(10,100 μmol·L-1) could inhibit L-type calcium channel current peak and the inhibitory rates were(9±7.5)%(n=5)and(28.6±8.5)%(n=5) individually.Conclusions Perforated patch clamp method has more stability and accuracy than ordinary whole-cell patch clamp technique in recording L-type calcium channel current;dehydrocorydaline can suppress L-type calcium channel in a concentration-dependent manner.