This paper had studied the anti-fatigue effects of the anti-fatigue liquid to complex Chinese traditional herbs on the swimming mice by animals.The result showed,blood lactate,lactic dehydrogenase,blood urea and superoxide dismutase and malondialdehyde's concentration of targets and the control group had obvious changes(P 0.01),and had a very significant difference.The results indicated that the anti-fatigue effect of the complicated Chinese side is very significant.