Optimum Technique of Extracting Total Saponins from Alfalfa by Supersonic Wave

Obiective:To optimize the extraction technique for total saponins from alfalfa by supersonic wave,the single factor tests and response surface method were used.Method:The factors including supersonic wave time,supersonic temperature,supersonic power,and the ratio of bittermelon to solvent,were studied.The best extraction method was found through the response surface method.Result:The reponse surface method with 4 factors and levels was adopted.The results showed that the optimun conditions were that supersonic wave time of 22 min,extracting temperature of 43℃,supersonic power of 403W,and the ratio of bittermelon to solvent of 1∶51(g/ml).Under such conditions the extraction rate of total saponins could be up to 4.53% at one-time extraction.