Determination of Thiamphenicol,Florfenicol and Florfenicol Amine in Lyophilized Royal Jelly Powder by HPLC

Objective To establish the high performance liquid chromatography (HPLC) method for the determination of thiamphenicol(TAP),florfenicol(FF)and florfenicol amine(FFA)in Lyophilized royal jelly powder.Method The analytes were extracted by ethyl acetate-acetonitrile-ammonia,purified by gel permeation chromatography(GPC) and detected by fluorescence detector.Results The average recoveries and relative standard deviations(RSDs)for the analyts were in the range of 84.6%-99.1%and 6.02%-9.81% (n=6),respectively.Good linearities were obtained in the concentration ranging from 0.20-5.0 mg/L for TAP and FF,and 0.10-4.0 mg/L for FFA.The limits of detection were 0.025mg/kg for TAP and FF,and 0.015 mg/kg for FFA.Conclusion The method is rapid,sensitive and can meet the needs for detection of thiamphenicol,florfenicol and florfenicol amine in lyophilized royal jelly powder.