Preparation of glycolic acid by biotransformation

Objective:Study on transformation technology of ethylene glycol to glycolic acid by Gluconobater oxydans.Methods:Biotransformation from ethylene glycol to glycolic acid by G.oxydans was optimized with single factor experiments and further study with orthogonal experiment.Results: the optimum conditions for ethylene glycol transformation were as follows:concentration of resting cells 12.6%,ethylene glycol 5.6%,pH 5.84, 48h.Transformation rate reached 60.886%under the optimum conditions.Conclusion:biotransformation of ethylene glycol to glycolic acid by G. oxydans is practicable.