The drought tolerance of 14 ecotypes of Vetiveria zizanioides germplasm resources was studied by several physiological indexes such as water-holding capacity in leaves,electrical conductivity rate,free proline contents and soluble sugar content. The results showed that obvious difference of drought tolerance existed in each ecotype of Vetiveria zizanioides,the ecotypic materials of Lilongwe,Karnataka and Parit buntar had the excellent ability on drought tolerance,and would be the original breeding material of variety with great drought tolerance.