Formation of the Transgenic Arabidopsis Plant with Two MYB Genes Tagging the Fusion Protein and Its Identification

[Objective] The downstream genes regulated by the MYB gene: At3g11280 and At5g05790,were developed through the experiment in chromosome immunoprecipitation(ChIP).[Method] Based on the construction of the plant expression vectors tagging fusion protein,the transgenic Arabidopsis plants were produced after these vectors were introduced into the plants by means of the agrobacterium-mediated transformation,which expression in transgenic plants was detected by Western blotting method.[Result] The transgenic efficiency of Arabidopsis plants was very high and more than 20 transgenic plants were produced under the transformation of four-tagged protein fusion vectors.The Western blotting analysis result from four random 8 T1 plants of four types of transgenic plants showed that the positive plants could be founded in the four types of transgenic plants.The band of fusion protein was about 30 kD and it can be seen that the size of displaying band was consistent with that of predicted protein after the band with color signal was compared with the Marker.[Conclusion] These transgenic plants expressing fusion protein would be the important material for ChIP experiment.