Review of Yarrowia lipolytica expression system

The ascomycetous yeast Yarrowia lipolytica is a representative one of non-conventional yeasts.An extensive description of assimilated substrates has been done.Yarrowia lipolytica has many characteristics,for example,it secrets large amounts of metabolites when use cheap substrates.A strong industrial interest arose from the fact that strains of this species were able to use normal paraffin as sole carbon source.Yarrowia lipolytica was developed into a new yeast expression system in 1990 s.It has been used to express about 42 heterologous proteins successfully.The characteristic expression system of Yarrowia lipolytica was reviewed,which is useful instrument for the investigation of heterologus protein secretion and regulation at transcriptional and translational level.