MYB公司
生物
拟南芥
非生物胁迫
拟南芥
苹果属植物
植物
细胞生物学
转录因子
基因
突变体
生物化学
作者
Ruigang Wu,Yi Wang,Ting Wu,Xuefeng Xu,Zhenhai Han
标识
DOI:10.1080/14620316.2017.1373038
摘要
MYB (v-myb avian myeloblastosis viral oncogene homolog) transcription factors (TFs) participate in the abiotic stress response in several plant species. In this study, we cloned and characterised MdMYB44 from apple (Malus domestica). Phylogenetic analysis showed that the MdMYB44 protein was highly homologous to Arabidopsis thaliana MYB44. In addition, MdMYB44 localised to the nuclei in transformed onion epidermal cells and showed transcriptional activation activity in a yeast one-hybrid assay. MdMYB44 was expressed in the stem, leaves, roots, flowers, and fruits, with the highest transcript level being observed in the leaves. Quantitative PCR analysis demonstrated that MdMYB44 expression was down-regulated by cold, osmotic, and salt stresses. Furthermore, stable overexpression of MdMYB44 in transgenic apple calli remarkably alleviated their tolerance to high salinity and cold, and relative conductivity and malondialdehyde accumulation under salt and cold treatments were significantly higher than those in wild-type control calli. Therefore, our findings provide evidence that MdMYB44 is involved in susceptibility to cold and salt stress in apple calli and indicate MdMYB44 to negatively regulate plant abiotic stress defence.
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