This paper reports on the reaction of Bacillus Licheniformis on the inhibitor variations to obtain a clear understanding of the relationship between Bacillus Licheniformis and the factor of physics and chemistry.The result shows that the inhibition on the growth of Bacillus Licheniformis is obvious when the pH is 7.0,the density of cephalospor is 0.5 g/L,the density of penicillin is 1 g/L and the density of streptomycin is 0.5 g/L. This provides a sicentific basis for using the preparation of the inhibitor of Bacillus Licheniformis.