The influence of activating agent on spore germination rate of Aspergillus oryzae.

Two strains of koji-making can make up for the lack of enzymes from Aspergillus oryzae,but germination time of Aspergillus oryzae spore is different from that of Aspergillus niger,which limits the practical application of two strains of koji-making in soy sauce production.The spore germination time of Aspergillus niger and Aspergillus oryzae was different under the same culture conditions.Spore germination rate of Aspergillus oryzae could be speeded up by adding activating agent,the optimal concentration of activating agent was 0.4%.The spores of Aspergillus oryzae and Aspergillus niger were cultured under different temperatures with this concentration,the results showed that spore germination rate of Aspergillus oryzae was almost the same as that of Aspergillus niger when cultured at 38 ℃ for 4.5 h,respectively reached 72% and 73%.