Microsatellite marker is a type of DNA marker which is used to detect DNA diversity in recent years. Due to the large of number, wide distribution, abundant polymorphic alleles, easy detection, it is used in high frequency. In this article, genetic diversity in Suzhong pig was analyzed by four microsatellite markers. Results showed that this four microsatellite markers had very high genetic diversity in Suzhong pig. Heterozygosity range was from 0.5962 to 0.8823, polymorphism information content from 0.5146 to 0.8709, effective numberofalleles from 2.4768 to 8.4935. Four micro- satellite markers used in this article can be adopted in marker assisted selection of genetic diversity in Suzhong pig.