打字
人类白细胞抗原
遗传学
生物
等位基因
序列(生物学)
计算生物学
聚合酶链反应
基因
抗原
作者
Michael Bunce,Ben Passey
出处
期刊:Methods in molecular biology
日期:2013-01-01
卷期号:: 147-159
被引量:17
标识
DOI:10.1007/978-1-62703-493-7_8
摘要
HLA typing by sequence-specific primers (PCR-SSP) is a commonly used technique in HLA typing in which multiple pairs of cis-located allele-specific primers are used to determine the alleles present in a given DNA sample. Although the technique is around two decades old, it still offers a relatively straight forward way of typing and has the benefit of using commonly available laboratory equipment. Here we describe the background of the PCR-SSP, how to design and validate reactions, and common problems that arise.
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