Simultaneous determination of six aflatoxins contaminants in peanut and their derivative products by ultra-performance liquid chromatography tandem mass spectrometry

Objective:A new separation and quantification method utilizing liquid chromatography under ultra-high-pressure in combination with tandem mass spectrometry(MS/MS) has been developed for simultaneous determination of the six aflatoxins(B1,B2,G1,G2,M1,M2) in peanuts and their derivative products.Methods:Homogenized samples were sequentially extracted by 84%%(v/v) of acetonitrile aqueous solution,cleaned up with self-made SPE columns,centrifuge,evaporate,re-dissolve and filtration.After injection,the analysis was performed on acquity UPLC HSS T3 column(100 mm×2.1 mm,1.8 μm particle size;Waters,Milford,MA,USA)using a mobile phase consisting of(A) water containing 0.1% formic acid and(B) acetate/methanol(50/50) with gradient elution.Results:The calibration curves were linear over the range of 0.08~10.0 μg/kg for aflatoxin B1,0.4~20.0 μg/kg for aflatoxin B2,1.0~20.0 μg/kg for aflatoxin G1,0.4~20.0 μg/kg for aflatoxin G2,0.4~20.0 μg/kg for aflatoxin M1,1.0~20.0 μg/kg for aflatoxin M2,respectively.The average recoveries ranged from 75.0%~86.9% with RSD≤9.4%.Conclusion:The twenty peanuts and their derivative products samples from different batches and different areas are analyzed by the established method.The results indicated that the established method is sensitive enough to meet the European regulations concerning aflatoxins,it can be applied as a rapid and reliable method for the authentication and quality evaluation of the aflatoxins in the peanuts and their derivative products.At the same time,aflatoxin M1 and M2 are detected in the peanuts and their derivative products for the first time.