Expression of NeuN in rat brain at different developmental stages

Objective To observe the expression of NeuN in rat brain at different developmental stages.Methods Immunohistochemistry and Western blot were applied to determine expression of NeuN in brain of SD rats at different developmental stages.Results At P0,the neurons of pallium and hippocampus were weakly stained by NeuN.To the development going on,the stain turned out stronger,especially in P15.which was similar to that in P30 and P90.The results of Western blot showed the expression of NeuN in rat brain was gradually increased with the development of rat brain,reaching peak at P30.Conclusion The expression of NeuN starts in rat brain before the birth and nearly be mature in P15.The development of grey matter is superior to white matter.