The pathogenicity for 13 strains of Alternaria alternata were studied,which were collected and isolated from different regions in Chongqing,were used to inoculate the detached leaves with suspension liquid and cultured properties were studied on PDA.The pathogenicity differentiation was shown by severity of the brown spots.Of the strains studied,3 appeared to be relatively powerful virulence,with a disease index of 686,632 and 618,respectively;2 were moderately virulence,with a disease index of 570 and 556,respectively,one showed relatively weak virulence,with a disease index of 229,5 were weak virulence,with disease indexes of 49,56,97,146 and 48,respectively,the other 2 were nonvirulent,and the most virulent strain was not found.The results indicated the ability of penetrating detached leaves and growth and their pathogenicity had a certainly common trend.The strains differing in pathogenicity existed in different tobaccogrowing area,and even strains with different virulence were found to be present in the same plot.The cultured properties of 13 strains were the same as that of the other reported strains,and the pathogenicity of strains was found to be related to their performance under cultured conditions.