Molecular mechanism of apoptosis in A_(2780) cisplatin-resistant human ovarian cancer cell line promoted by H_(13)

To investigate the cytotoxicity and proapoptotic activity of H_(13) on cisplatin-resistant human ovarian cancer cell lines A_(2780) and its mocular mechanisms.The cytotoxicity of H_(13) on A_(2780) was monitered using MTT assay; Flow cytometry was employed to observe the apoptosis of A_(2780) induced by H_(13);Western blot were used to determine the expression of p53 and caspase-3.H_(13) had strong cytotoxicity on A_(2780) cell invitro,and the cytotoxicity was dose-dependent. H_(13)-treated cells demonstrated apoptosis property while the apoptotic rate was increased from 5.7% to 20.16%.The expression of p53 were upregulated and the expression of caspase-3 in the course of A_(2780) apoptosis when treated with H_(13).The cytotoxicity and proapoptosis activity of H_(13),which is significantly observed in cisplatin-resistant human ovarian cancer,may be related to the increased p53 expression and caspase-3 expression.