胸腺基质淋巴细胞生成素
间质细胞
树突状细胞
生物
免疫学
癌症研究
医学
细胞生物学
免疫系统
作者
Laura Kummola,Zsuzsanna Ortutay,Xi Chen,Stéphane Caucheteux,Sanna Hämäläinen,Saara Aittomäki,Ryoji Yagi,Jinfang Zhu,Marko Pesu,William E. Paul,Ilkka Junttila
出处
期刊:Journal of Immunology
[American Association of Immunologists]
日期:2017-04-13
卷期号:198 (10): 3909-3918
被引量:10
标识
DOI:10.4049/jimmunol.1600753
摘要
Abstract Thymic stromal lymphopoietin (TSLP) and IL-7 are related cytokines that mediate growth and differentiation events in the immune system. They signal through IL-7Rα–containing receptors. Target cells of TSLP in Th2 responses include CD4 T cells and dendritic cells (DCs). Although it has been reported that expression of TSLP receptor (TSLPR) on CD4 T cells is required for OVA-induced lung inflammation, DCs have also been shown to be target cells of TSLP. In this study, we show that murine ex vivo splenic DCs are unresponsive to TSLP, as they fail to phosphorylate STAT5, but in vitro overnight culture, especially in presence of IL-4, renders DCs responsive to both TSLP and IL-7. This induced responsiveness is accompanied by dramatic upregulation of IL-7Rα on DCs with little change in expression of TSLPR or of γc. In splenic DCs, the induction of IL-7Rα occurs mainly in CD8− DCs. In vivo, we found that IL-4 has a differential regulatory role on expression of IL-7Rα depending on the cell type; IL-4 decreases IL-7Rα expression on CD4 T cells whereas it upregulates the expression on DCs. Our results indicate that the induction of IL-7Rα expression on DCs is critical for TSLP responsiveness and that IL-4 can upregulate IL-7Rα on DCs.
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