类有机物
低温保存
低温保护剂
低温生物学
细胞生物学
生物医学工程
生物
化学
胚胎
医学
作者
Seulgi Lee,Jin Kim,Jin Seok,Min Woo Kim,Jiyoung Rhee,Guofeng Song,Shinhye Park,Suemin Lee,Yunkyung Jeong,Hyung Min Chung,C‐Yoon Kim
标识
DOI:10.1002/biot.202300311
摘要
Abstract Beyond single cell two‐dimensional (2D) culture, research on organoids that can mimic human organs is rapidly developing. However, there are still problems in commercialization and joint research using organoids due to the lack of technology to safely store organoids. Since organoids are 3D complex structures with a certain size (0.1–5 mm) beyond the size of cells, the conventional cell‐level cryopreservation method using cryoprotectant (CPA) cannot overcome the damage caused by volume change due to osmotic pressure difference and ice nucleation. Herein, we attempted to solve such limitations by applying a nanowarming system using CPA with high cell permeability and Fe 3 O 4 nanoparticles. By performing beat rate measurement, histological analysis, contractility analysis, and multi‐electrode array, it was verified that the developed method could significantly improve functional recovery and survival of heart organoids after freezing and thawing. In this study, we demonstrated a successful organoid cryopreservation method based on a Fe 3 O 4 nanowarming system. The developed technology will provide clues to the field of tissue cryopreservation and spur the application of organoids.
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