Comparative study on alleviating effect of kiwi berry (Actinidia arguta) polysaccharide and polyphenol extracts on constipated mice

Kiwi berry (Actinidia arguta) is beneficial for relieving constipation, but the mechanism of easing constipation is still unknown. The alleviating effects of kiwi berry polysaccharide and polyphenol extracts on loperamide induced constipation were studied. Administration with polysaccharide extract of kiwi berry in loperamide-induced constipation mice distinctly decreased the body weight gain by 124.0%, the number and the water content of feces was decreased by 152.4% and 107.0% respectively, gastrointestinal (GI) transit rate was decreased by 39.5% and the time to the first dark stool was largen by 56.2% as compared with those in the loperamide group, respectively. The levels of excitability neurotransmitters were increased, and the inhibitory neurotransmitter was decreased in the kiwi berry extracts groups compared with the loperamide group. The levels of aquaporins were decreased to ameliorate constipation. Moreover, kiwi berry extracts can protect colon smooth muscle cells from apoptosis and help to restore colon health. Interstitial cells of Cajal (ICC) and animal experiments suggested that kiwi berry extracts can up-regulate the expression levels of stem cell factors (SCF)/c-kit protein. Kiwi berry can remodel the structure of microbial communities. All findings suggest that kiwi berry polysaccharide and polyphenol especially its polysaccharide extract, can effectively alleviate constipation induced by loperamide. Kiwi berry is a promising food supplement that can be used to relieve constipation.