Photothermal-enhanced multifunctional nanozyme for regulate GSH and H2O2 in tumor for improving catalytic therapy

Abstract Nanozyme-based catalytic therapy have been limited by poor catalyic performance, insufficient endogenous H2O2 concentration and high concentrations of GSH in tumor microenvironment. Herein, bimetallic MOFs were synthesized, loaded with glucose oxidase and then modified with polydopamine to obtain photothermal-enhanced nanozyme (Zr/Ce-MOFs/GOx/PDA) with GSH and H2O2 regulating properties for tumor catalytic therapy. The results showed that Zr/Ce-MOFs/GOx/PDA nanozyme exhibits intrinsic peroxidase-like and GPx-like activities , which can not only provide H2O2 in situ and decompose H2O2 into ·OH, but also eliminate GSH to protect ·OH, which can promote catalytic activities of Zr/Ce-MOFs/GOx/PDA. Besides, the Zr/Ce-MOFs/GOx/PDA with photothermal performance can further promote the catalytic activities, and the photothermal efficiency of Zr/Ce-MOFs/GOx/PDA nanozyme were 26.2%. The potential anti-tumor effect of Zr/Ce-MOFs/GOx/PDA was proved by in vitro 4T1 and in vivo 4T1-tumor-bearing mice models. It is believed that the photothermal-boosted multifunctional nanozyme-based tumor treatment strategy is a prospective method to improve therapy of tumor.