羟基烷酸
淀粉
盐单胞菌属
化学
食品科学
大肠杆菌
生物化学
极端微生物
淀粉酶
酶
嗜盐菌
细菌
微生物学
生物
嗜热菌
基因
遗传学
16S核糖体RNA
作者
Yuzhong Liu,Xueqi Song,Wenhao Yang,Meng-Ru Wang,Guoli Lian,Zhengjun Li
标识
DOI:10.1016/j.ijbiomac.2024.129838
摘要
A novel α-amylase Amy03713 was screened and cloned from the starch utilization strain Vibrio alginolyticus LHF01. When heterologously expressed in Escherichia coli, Amy03713 exhibited the highest enzyme activity at 45 °C and pH 7, maintained >50 % of the enzyme activity in the range of 25–75 °C and pH 5–9, and sustained >80 % of the enzyme activity in 25 % (w/v) of NaCl solution, thus showing a wide range of adapted temperatures, pH, and salt concentrations. Halomonas bluephagenesis harboring amy03713 gene was able to directly utilize starch. With optimized amylase expression, H. bluephagenesis could produce poly(3-hydroxybutyrate) (PHB), poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P34HB). When cultured for PHB production, recombinant H. bluephagenesis was able to grow up to a cell dry weight of 11.26 g/L, achieving a PHB titer of 6.32 g/L, which is the highest titer that has been reported for PHB production from starch in shake flasks. This study suggests that Amy03713 is an ideal amylase for PHA production using starch as the carbon source in H. bluephagenesis.
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