动细胞
染色体分离
细胞生物学
微管
生物
有丝分裂
着丝粒
主轴装置
极光激酶B
主轴杆体
细胞分裂
染色体
遗传学
细胞
基因
作者
Kyle Muir,Christopher Batters,Tom Dendooven,Jing Yang,Ziguo Zhang,Alister Burt,David Barford
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2023-12-08
卷期号:382 (6675): 1184-1190
被引量:2
标识
DOI:10.1126/science.adj8736
摘要
Kinetochores couple chromosomes to the mitotic spindle to segregate the genome during cell division. An error correction mechanism drives the turnover of kinetochore-microtubule attachments until biorientation is achieved. The structural basis for how kinetochore-mediated chromosome segregation is accomplished and regulated remains an outstanding question. In this work, we describe the cryo–electron microscopy structure of the budding yeast outer kinetochore Ndc80 and Dam1 ring complexes assembled onto microtubules. Complex assembly occurs through multiple interfaces, and a staple within Dam1 aids ring assembly. Perturbation of key interfaces suppresses yeast viability. Force-rupture assays indicated that this is a consequence of impaired kinetochore-microtubule attachment. The presence of error correction phosphorylation sites at Ndc80-Dam1 ring complex interfaces and the Dam1 staple explains how kinetochore-microtubule attachments are destabilized and reset.
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