电化学发光
量子点
检出限
发光
共轭体系
材料科学
化学
分析化学(期刊)
纳米技术
光电子学
色谱法
聚合物
复合材料
作者
Wenrui Zhao,Yaohua Xu,Tianfang Kang,Xiao Zhang,Hao Líu,Anjie Ming,Shuiyuan Cheng,Feng Wei
标识
DOI:10.1016/j.bios.2020.112102
摘要
A molecularly imprinted magnetic sensor with electroluminescent tags (MIP-ECL sensor) was developed for ultrasensing diethylstilbestrol (DES). A strategy is exploited to enhance ECL emission of the [Ru(bpy)3]2 +-tripropyl amine (TPrA) system by [email protected] quantum-dots (QDs) through energy transfer. Magnetically molecularly imprinted nanoparticles (MMIPs NPs) based on Fe3O4@SiO2 carriers are artificial, easily reproducible, and could replace easily inactivated first antibodies for capturing more DES molecules. Functionalized bio-conjugates of single [email protected] ([email protected]) are for the first time loaded on signal labels of Ru(bpy)32 +-doped silica nanocomposites ([email protected]2) for signal amplification. The final bio-conjugated signal probes are denoted as Ab-DES/[email protected]–[email protected]2. MMIPs beads that have captured antigens are bio-conjugated with antibody-labeled luminescent probes by specific immunoreactive reaction, and then the luminescent immunocomplex generates ECL signal on the magnetic electrode. The logarithm of ECL intensities depend linearly on the logarithm of DES concentrations in the range from 4.8 × 10− 4 to 36.0 nM with a detection limit of 0.025 pM. This novel assay is much more sensitive than other MIP sensors, and achieves lower cost and more enhanced stability than other immunosensors. The sensor is significantly potential and has been applied to DES detection in actual environment.
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