Identification wild and cultivated licorice by multidimensional analysis

Licorice is known as a botanical source in medicine and a sweetening agent in food products. Commercial licorice is from the source of wild and cultivated G. uralensis. It was recognized that the material basis of wild and cultivated licorice is different. This study systematically investigated the difference between them by multidimensional analysis technology. The results showed that the content of starch grain, total dietary fibre (TDF), and 11 secondary metabolite components was significantly different in wild and cultivated licorice. principal component analysis (PCA) and orthogonal partial least square (OPLS-DA) analyses showed that the wild and cultivated licorice samples could be clearly separated based on the acquired data of microscopic, macromolecular substance and secondary metabolite analysis. The main markers were starch grain, isoliquiritin apioside, liquirtin apioside and TDF. Additionally, NIR spectroscpy combined with PLS-DA has demonstrated a suitable, fast and nondestructive methodology for authentication of wild and cultivated licorice.