医学
心肌梗塞
金标准(测试)
经皮冠状动脉介入治疗
内科学
生物标志物
心脏病学
免疫分析
注意事项
重症监护医学
病理
免疫学
生物
生物化学
抗体
作者
Wei Xu,Lin Wang,Hongjie Dai,Xuming Sun,Lin Huang,Housheng Su,Xunbin Wei,Chia Chun Chen,Jiatao Lou,Hongjie Dai,Kun Qian
标识
DOI:10.1038/s41467-020-15487-3
摘要
Abstract Cardiovascular diseases lead to 31.5% of deaths globally, and particularly myocardial infarction (MI) results in 7.4 million deaths per year. Diagnosis of MI and monitoring for prognostic use are critical for clinical management and biomedical research, which require advanced tools with accuracy and speed. Herein, we developed a plasmonic gold nano-island (pGold) chip assay for diagnosis and monitoring of MI. On-chip microarray analysis of serum biomarkers (e.g., cardiac troponin I) afforded up to 130-fold enhancement of near-infrared fluorescence for ultra-sensitive and quantitative detection within controlled periods, using 10 μL of serum only. The pGold chip assay achieved MI diagnostic sensitivity of 100% and specificity of 95.54%, superior to the standard chemiluminescence immunoassay in cardiovascular clinics. Further, we monitored biomarker concentrations regarding percutaneous coronary intervention for prognostic purpose. Our work demonstrated a designed approach using plasmonic materials for enhanced diagnosis and monitoring for prognostic use towards point-of-care testing.
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