表面活性蛋白D
收藏品
支气管肺泡灌洗
肺表面活性物质
表面活性蛋白A
肺泡蛋白沉积症
先天免疫系统
羊水
重组DNA
内质网
生物
过敏原
炎症
免疫系统
分子生物学
免疫学
肺
化学
过敏
细胞生物学
医学
生物化学
内科学
怀孕
胎儿
遗传学
基因
作者
Eswari Dodagatta-Marri,Asif S. Qaseem,Najmunisa Karbani,Anthony G. Tsolaki,Patrick Waters,Taruna Madan,Uday Kishore
出处
期刊:Methods in molecular biology
日期:2013-10-22
卷期号:: 273-290
被引量:11
标识
DOI:10.1007/978-1-62703-724-2_22
摘要
Surfactant protein SP-D is a multimeric collagenous lectin, called collectin. SP-D is a multifunctional, pattern recognition innate immune molecule, which binds in a calcium dependent manner to an array of carbohydrates and lipids, thus offering resistance to invading pathogens, allergen challenge, and pulmonary inflammation. SP-D is predominantly found in the endoplasmic reticulum of type 2 pneumocytes and in the secretory granules of Clara or non-ciliated bronchiolar cells. The highest expression of SP-D is observed in the distal airways and alveoli. There is also an extra pulmonary existence of SP-D. The common sources of native full-length human SP-D are bronchoalveolar lavage (BAL) washings from normal or preferably patients suffering from alveolar proteinosis who overproduce SP-D in the lungs. Amniotic fluid collected at the term during parturition is another reasonable source. Here, we describe a simple and rapid method of purifying native SP-D away from SP-A which is also present in the same source. We also describe procedures of expressing and purifying a recombinant fragment of human SP-D (rhSP-D) comprising trimeric neck and carbohydrate recognition domains that has been shown to have therapeutic effects in murine models of allergy and infection.
科研通智能强力驱动
Strongly Powered by AbleSci AI