Optical skin biopsy using multimodal line-field confocal optical coherence tomography (LC-OCT)

Line-field confocal optical coherence tomography (LC-OCT) is an imaging technique based on a combination of confocal microscopy and OCT, allowing three-dimensional cellular-resolution imaging of the skin in vivo. We present the latest advances in LC-OCT to facilitate the use of the technique by dermatologists and improve the diagnosis and analysis of skin lesions. A video camera was incorporated into a handheld probe to acquire dermoscopic images in parallel with LCOCT images. A confocal Raman spectrometer was associated with a LC-OCT device to record morphological images of the skin in which points of interest can be subjected to molecular characterization.