Salting-Out Approach Is Worthy of Comparison with Ultracentrifugation for Extracellular Vesicle Isolation from Tumor and Healthy Models

纳米粒子跟踪分析 超离心机 分离(微生物学) 分析超速离心 细胞外小泡 流式细胞术 色谱法 盐析 化学 动态光散射 胞外囊泡 细胞生物学 计算生物学 微泡 生物化学 生物 纳米技术 分子生物学 材料科学 纳米颗粒 生物信息学 水溶液 物理化学 小RNA 基因
作者
Simona Serratì,Antonio J. Palazzo,Annamaria Lapenna,Helena Mateos,Antonia Mallardi,René Massimiliano Marsano,Alessandra Quarta,Mario Del Rosso,Amalia Azzariti
出处
期刊:Biomolecules [MDPI AG]
卷期号:11 (12): 1857-1857 被引量:3
标识
DOI:10.3390/biom11121857
摘要

The role of extracellular vesicles (EVs) has been completely re-evaluated in the recent decades, and EVs are currently considered to be among the main players in intercellular communication. Beyond their functional aspects, there is strong interest in the development of faster and less expensive isolation protocols that are as reliable for post-isolation characterisations as already-established methods. Therefore, the identification of easy and accessible EV isolation techniques with a low price/performance ratio is of paramount importance. We isolated EVs from a wide spectrum of samples of biological and clinical interest by choosing two isolation techniques, based on their wide use and affordability: ultracentrifugation and salting-out. We collected EVs from human cancer and healthy cell culture media, yeast, bacteria and Drosophila culture media and human fluids (plasma, urine and saliva). The size distribution and concentration of EVs were measured by nanoparticle tracking analysis and dynamic light scattering, and protein depletion was measured by a colorimetric nanoplasmonic assay. Finally, the EVs were characterised by flow cytometry. Our results showed that the salting-out method had a good efficiency in EV separation and was more efficient in protein depletion than ultracentrifugation. Thus, salting-out may represent a good alternative to ultracentrifugation.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
蜂蜜兑多了完成签到 ,获得积分10
刚刚
1秒前
chao发布了新的文献求助20
1秒前
yydsyyd完成签到 ,获得积分10
2秒前
2秒前
gzgljh完成签到,获得积分10
4秒前
5秒前
MZ发布了新的文献求助10
6秒前
6秒前
匆匆完成签到 ,获得积分10
7秒前
喜悦的冷松完成签到,获得积分10
8秒前
Chris发布了新的文献求助50
9秒前
好好完成签到 ,获得积分10
10秒前
瘦瘦的乐曲关注了科研通微信公众号
11秒前
13秒前
14秒前
15秒前
16秒前
17秒前
玲玲发布了新的文献求助10
18秒前
大聪明发布了新的文献求助10
20秒前
duxiao完成签到 ,获得积分10
23秒前
23秒前
锦瑟完成签到,获得积分10
24秒前
26秒前
27秒前
负责人生发布了新的文献求助10
28秒前
28秒前
ant完成签到,获得积分10
29秒前
29秒前
FashionBoy应助科研通管家采纳,获得10
29秒前
英姑应助科研通管家采纳,获得10
29秒前
上官若男应助科研通管家采纳,获得10
29秒前
NexusExplorer应助科研通管家采纳,获得10
30秒前
李健应助科研通管家采纳,获得10
30秒前
赘婿应助科研通管家采纳,获得10
30秒前
30秒前
逗逗不觉完成签到 ,获得积分10
30秒前
FashionBoy应助科研通管家采纳,获得10
30秒前
zijin应助科研通管家采纳,获得10
30秒前
高分求助中
请在求助之前详细阅读求助说明!!!! 20000
The Three Stars Each: The Astrolabes and Related Texts 900
Yuwu Song, Biographical Dictionary of the People's Republic of China 700
Bernd Ziesemer - Maos deutscher Topagent: Wie China die Bundesrepublik eroberte 500
A radiographic standard of reference for the growing knee 400
Glossary of Geology 400
Additive Manufacturing Design and Applications 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 有机化学 工程类 生物化学 纳米技术 物理 内科学 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 电极 光电子学 量子力学
热门帖子
关注 科研通微信公众号,转发送积分 2474094
求助须知:如何正确求助?哪些是违规求助? 2139091
关于积分的说明 5451650
捐赠科研通 1863070
什么是DOI,文献DOI怎么找? 926308
版权声明 562817
科研通“疑难数据库(出版商)”最低求助积分说明 495512