环介导等温扩增
荧光
插层(化学)
石墨烯
生物分析
聚合
信号(编程语言)
分子信标
纳米技术
DNA
化学
组合化学
生物物理学
核酸
滚动圆复制
材料科学
寡核苷酸
聚合酶
计算机科学
生物化学
聚合物
有机化学
生物
物理
程序设计语言
量子力学
作者
Xiao Zhu,Xiaoming Zhou,Da Xing
标识
DOI:10.1002/chem.201204605
摘要
A label-free approach with multiple enhancement of the signal for microRNA detection has been introduced. The key idea of this strategy is achieved by taking advantage of a novel graphene oxide (GO)/intercalating dye based fluorescent hairpin probe (HP) and an isothermal polymerization reaction. In this paper, we used microRNA-21 (mir-21) as the target to examine the desirable properties of this assay. When the target, as a "trigger", was hybridized with the HP and caused a conformation change, an efficient isothermal polymerization reaction was activated to achieve the first step of the "signal" amplification. After incubation with the platform of GO/intercalating dye, the formed complex of DNA interacted with the high-affinity dye and then detached from the surface of the GO, a process that was accompanied by distinguishable fluorescence recovery. Further signal enhancement has been accomplished by a mass of intercalating dye inserting into the minor groove of the long duplex replication product. Due to the efficient and multiple amplification steps, this approach exerted a substantial enhancement in sensitivity and could be used for rapid and selective detection of Mir-21 at attomole levels. Proof-of-concept evidence has been provided for the proposed cost-effective strategy; thus, this strategy could expand the application of GO-material-based bioanalysis for nucleic acid studies.
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