The bioconversion of fruit wastes into single cell protein production has the potential to solve the worldwide food protein deficiency by obtaining an economical product for food and feed. Using food processing leftovers in the production of single cell protein as substrate would alleviate pollution. Pineapple peels contain significant quantities of carbohydrates, which can be used as cheap raw materials for production of commercially important products through fermentation. The increased concentration of pineapple hydrolysate enhanced the biomass yield and the protein formation within the yeast cells. Lower carbon utilization by the two yeast strains occurred in the waste containing media, as compared to control, increasing the economic value of the waste obtained after 7-day fermentation. The present finding helps in SCP production from cheap, inexpensive agro waste material.