离子载体
卵母细胞
胚泡
原核
人类受精
卵母细胞激活
男科
劈理(地质)
胚胎
生物
胚胎发生
化学
合子
医学
内科学
细胞生物学
解剖
钙
古生物学
断裂(地质)
作者
Omar Shebl,Philip Sebastian Trautner,Sabine Enengl,Elisabeth Reiter,Christina Allerstorfer,T Rechberger,Peter Oppelt,Thomas Ebner
标识
DOI:10.1007/s10815-021-02338-3
摘要
To evaluate whether ionophore application at the oocyte stage changes the morphokinetics of the associated embryos in cases of artificial oocyte activation.In a prospective sibling oocyte approach, 78 ICSI patients with suspected fertilization problems had half of their MII-oocytes treated with a ready-to-use ionophore (calcimycin) immediately following ICSI (study group). Untreated ICSI eggs served as the control group. Primary analyses focused on morphokinetic behavior and the presence of irregular cleavages. The rates of fertilization, utilization, pregnancy, and live birth rate were also evaluated.Ionophore-treated oocytes showed a significantly earlier formation of pronuclei (t2PNa) and a better synchronized third cell cycle (s3) (P < .05). The rate of irregular cleavage was unaffected (P > .05). Ionophore treatment significantly improved the overall rates of fertilization (P < .01) and blastocyst utilization (P < .05).Ionophore application does not negatively affect cleavage timing nor is it associated with irregular cleavage.
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