适体
生物传感器
肌钙蛋白I
检出限
心肌梗塞
肌钙蛋白
生物医学工程
生物标志物
金标准(测试)
医学
心脏病学
材料科学
内科学
纳米技术
化学
色谱法
分子生物学
生物
生物化学
作者
Masoud Negahdary,Mostafa Behjati-Ardakani,N. Sattarahmady,H. Heli
标识
DOI:10.31661/jbpe.v8i2.930
摘要
Background: Acute myocardial infarction (MI) accounts for one third of deaths. Cardiac troponin I (TnI) is a reliable biomarker of cardiac muscle tissue injury and is employed in the early diagnosis of MI.Objectives: In this study, a molecular method is introduced to early diagnosis of MI by rapid detection of TnI.Materials and Methods: The detection method was based on electrochemical aptasensing, being developed using different methods and evaluation steps. A gold electrode was used as a transducer to successful immobilize 76base aptamer to fabricate a TnI biosensor.Results: The designed aptasensor could detect TnI in a range of 0.03 to 2.0 ng mL-1 without using any label, pre-concentration or amplification steps. The limit of detection was attained as 10 pg mL-1 without significant trouble of interfering species. The TnI biosensor demonestrated a stable, regenerative and reproducible function. 89 human samples were used to evaluate the performance of the TnI biosensor, and it represented 100% and 81%, diagnostic sensitivity and specificity, respectively.Conclusions: This aptasensor may be used as an applicable tool in the future of early medical diagnosis of MI.
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