生物传感器
体内
微透析
离体
生物医学工程
抗生素
抗菌剂
化学
色谱法
药理学
材料科学
纳米技术
医学
生物化学
生物
生物技术
有机化学
作者
Sally A. N. Gowers,David M. Freeman,Timothy M. Rawson,Michelle Rogers,Riley Wilson,Alison Holmes,Anthony E. G. Cass,Danny O’Hare
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2019-04-05
卷期号:4 (4): 1072-1080
被引量:92
标识
DOI:10.1021/acssensors.9b00288
摘要
Antimicrobial resistance poses a global threat to patient health. Improving the use and effectiveness of antimicrobials is critical in addressing this issue. This includes optimizing the dose of antibiotic delivered to each individual. New sensing approaches that track antimicrobial concentration for each patient in real time could allow individualized drug dosing. This work presents a potentiometric microneedle-based biosensor to detect levels of β-lactam antibiotics in vivo in a healthy human volunteer. The biosensor is coated with a pH-sensitive iridium oxide layer, which detects changes in local pH as a result of β-lactam hydrolysis by β-lactamase immobilized on the electrode surface. Development and optimization of the biosensor coatings are presented, giving a limit of detection of 6.8 μM in 10 mM PBS solution. Biosensors were found to be stable for up to 2 weeks at -20 °C and to withstand sterilization. Sensitivity was retained after application for 6 h in vivo. Proof-of-concept results are presented showing that penicillin concentrations measured using the microneedle-based biosensor track those measured using both discrete blood and microdialysis sampling in vivo. These preliminary results show the potential of this microneedle-based biosensor to provide a minimally invasive means to measure real-time β-lactam concentrations in vivo, representing an important first step toward a closed-loop therapeutic drug monitoring system.
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