Optimizing the formulation of vinpocetine solid lipid nanoparticles by Box-Behnken experimental design

OBJECTIVE To develop and optimize the formulation of vinpocetine solid lipid nanoparticles.METHODS The vinpocetine solid lipid nanoparticles were prepared by melt-emulsion ultrasonication and low temperature-solidification methods.The formulation variables were optimized by‘Box-Behnken design(BBD)'with response surface methodology(RSM)of glyceryl behenate,fluronic F-68 and the ratio of drug to lipid as independent variables,and encapsulation efficiency and particle sizeas dependent variables.The optimized solid lipid nanoparticles was characterized for encapsulation efficiency,particle size,zeta potential,morphology,and in vitro drug release behavior of solid lipid nanoparticles was also studied.RESULTS For encapsulation efficiency,particle size,zeta potential of solid lipid nanoparticles were found to be(84.7±2.7)%,(196.6±23.4)nm and(-34.3±2.4)mV,respectively.The solid lipid nanoparticles were found to be small and spherical with smooth surface as seen in transmission electron microscopy.The in vitro release data proved that the drug release was steady during 24 h.CONCLUSION The Box-Behnken experimental design facilitates the process of vinpocetine solid lipid nanoparticles.