One bacterial strain degrading L -phosphiothricin (PPT) was isolated from soil sprayed with herbicide. The strain could grow on medium containing PPT as sole carbon source and resist PPT with concentration up to 2.7 g/L. In addition to general physiological and biochemical properties, the strain was identified by 16S rDNA sequence and systematic analysis. The results showed that 16S rDNA sequence of the strain had similarity of 99.3% with that of Enterobacter cancerogenus , suggesting that the strain belong to Enterobacter . The strain was nominated as Enterobacter sp. PPT. Fig 4, Tab 2, Ref 7