Study on Interaction between 1-(2-Pyridylazo)-2-Naphthol with Bovine Serum Albumin

The interaction between 1-(2-pyridylazo)-2-naphthol(PAN) with bovine serum albumin(BSA) was studied by using fluorescence spectroscopy combined with ultraviolet-visible absorption spectroscopy,and circular dichroism(CD) methods.The results showed that static quenching occurred through the formation of complex of PAN and BSA.The static quenching mechanism of PAN for BSA was confirmed by UV-visible absorption spectra.The thermodynamic parameters of the system were calculated according to Van't Hoff equation,and the results indicates that both of the ΔH and ΔS are less than zero,which indicates that the main interaction between PAN and BSA is hydrogen bonds and van der Waals force.Circular dichroism studies showed that the secondary structure of BSA had been changed,which is caused by PAN indicator.