化学
费斯特共振能量转移
核苷酸
荧光
立体化学
接受者
三环
分子
DNA
核酸
打字
生物化学
有机化学
遗传学
物理
基因
生物
量子力学
凝聚态物理
作者
Aya Hayai,Yusuke Maeda,Yoshihito Ueno
标识
DOI:10.1016/j.bmcl.2016.06.024
摘要
Here, we report the synthesis of a fluorescence resonance energy transfer (FRET)-based probe for single nucleotide polymorphism (SNP) typing. The probe contains a fluorescent tricyclic base, 8-amino-3-(2,3-dihydroxypropyl)imidazo[4′,5′:5,6]pyrido[2,3-d]pyrimidine, as a donor molecule and 7-diethylaminocoumarin-3-carboxylic acid as an acceptor molecule. FRET was observed between the donor and acceptor molecules on the probe. The identity of the target bases on DNA and RNA strands could be determined using the probe.
科研通智能强力驱动
Strongly Powered by AbleSci AI