癌变
癌症研究
基因敲除
安普克
PI3K/AKT/mTOR通路
腺癌
肺癌
信号转导
己糖激酶
生物
蛋白激酶B
癌症
下调和上调
糖酵解
医学
内科学
细胞生物学
基因
激酶
蛋白激酶A
内分泌学
遗传学
新陈代谢
作者
Xinyu Wang,Bowen Shi,Yue Zhao,Qijue Lu,Xiang Fei,Chaojing Lu,Chunguang Li,Hezhong Chen
标识
DOI:10.1186/s12935-020-01539-7
摘要
Hexokinase domain component 1 (HKDC1) plays an oncogenic role in certain types of cancer, such as lymphoma, liver cancer, and breast cancer. Previous bioinformatics study revealed that HKDC1 was significantly upregulated in lung adenocarcinoma (LUAD). However, its biological functions and potential mechanism in LUAD have not been studied.We performed bioinformatics analysis, quantitative real-time polymerase chain reaction (qRT-PCR), western blotting, immunohistochemistry, and a series of functional assays in vitro and in vivo to investigate the roles of HKDC1 in LUAD.We discovered that HKDC1 was highly expressed in LUAD tissues and cell lines, and the positive expression of HKDC1 was correlated with aberrant clinicopathological characteristics in LUAD patients. Furthermore, HKDC1 could serve as a prognostic predictor for LUAD patients. Overexpression of HKDC1 promoted proliferation, migration, invasion, glycolysis, EMT and tumorigenicity, whereas knockdown of HKDC1 produced the opposite functional effects. Mechanistically, HKDC1 could regulate the AMPK/mTOR signaling pathway to perform its biological function.Our findings suggest that HKDC1 plays an oncogenic role in LUAD. Targeting this gene may provide a promising therapeutic target to delay LUAD progression.
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