Stabilization of hydrochlorothiazide nanocrystals using fibroin

Nanocrystals of the poorly water soluble drugs is a promising strategy.To stabilize the drug nanocrystals, stabilizers are usually used; however, the use of common stabilizers is limited by weak stabilization effect and toxicological concerns for long-term treatment.The present work was aimed to investigate the potential of natural silk protein as novel stabilizer for nanocrystal of hydrochlorothiazide, which was a model drug.The nanocrystals of drug with hydrophobic protein stabilizer were prepared by antisolvent precipitation method.Prepared nanocrystals were evaluated for parameters like particle size, zeta potential, DSC, XRD, %crystallinity, SEM, drug content and in vitro dissolution test.Stabilization efficiency of nanocrystals was assessed by their % crystallinity for 3 months.Optimized batch R2 was shown smaller particle size, highest drug content and drug dissolution.Nanocrystals were shown the extended release due to coat of fibroin around the hydrochlorothiazide.From the % crystallinity study it was found that there was not significant change in the nanocrystals prepared using fibroin.So, it was concluded that the fibroin is good stabilizer for drug nanocrystals.