细胞生物学
运动性
糖酵解
罗亚
过剩3
内皮干细胞
细胞骨架
细胞迁移
焦点粘着
肌动蛋白解聚因子
细胞
厌氧糖酵解
肌动蛋白细胞骨架
生物
生物化学
葡萄糖转运蛋白
信号转导
新陈代谢
过剩1
内分泌学
胰岛素
体外
作者
David Wu,Devin L. Harrison,Teodora Szasz,Chih‐Fan Yeh,Tzu‐Pin Shentu,Angelo Y. Meliton,Ru‐Ting Huang,Zhong Zhou,Gökhan M. Mutlu,Jun Huang,Yun Fang
标识
DOI:10.1038/s42255-021-00390-y
摘要
Single-cell motility is spatially heterogeneous and driven by metabolic energy. Directly linking cell motility to cell metabolism is technically challenging but biologically important. Here, we use single-cell metabolic imaging to measure glycolysis in individual endothelial cells with genetically encoded biosensors capable of deciphering metabolic heterogeneity at subcellular resolution. We show that cellular glycolysis fuels endothelial activation, migration and contraction and that sites of high lactate production colocalize with active cytoskeletal remodelling within an endothelial cell. Mechanistically, RhoA induces endothelial glycolysis for the phosphorylation of cofilin and myosin light chain in order to reorganize the cytoskeleton and thus control cell motility; RhoA activation triggers a glycolytic burst through the translocation of the glucose transporter SLC2A3/GLUT3 to fuel the cellular contractile machinery, as demonstrated across multiple endothelial cell types. Our data indicate that Rho-GTPase signalling coordinates energy metabolism with cytoskeleton remodelling to regulate endothelial cell motility.
科研通智能强力驱动
Strongly Powered by AbleSci AI