One-step extraction and simultaneous quantitative fluorescence immunochromatography strip for AFB1 and Cd detection in grain

AFB1 and heavy metal Cd are two common pollutants during grain storage. The rapid detection of grains before they enter the granary is particularly important. Hence, rapidly, accurately, and sensitively screening contaminated grains, simplifying the detection process, and reducing detection costs are necessary. In this study, linear ranges of time-resolved fluorescence microsphere - immunochromatographic test strip (TRFM-ICTS) detection were 0.01-30 ng/mL (AFB1) and 0.01-60 ng/mL (Cd), and the IC50 values were 0.536 ng/mL (AFB1) and 3.331 ng/mL (Cd). In the TRFM-ICTS sample addition experiment, the recovery rates were all between 90% and 110%. The coefficient of variation was less than 8% in the actual sample detection process of grain. We have established a one-step extraction method for AFB1 and Cd in grains to achieve simultaneous detection in one extraction. In addition, TRFM-ICTS could be stored for at least 12 months, providing technical support for the realization of commercial production.